Illumina  簇生成和測(cè)序試劑

Illumina  簇生成和測(cè)序試劑

上海易匯生物科技有限公司于17年正式銷(xiāo)售Illumina公司基因芯片、二代測(cè)序NGS測(cè)序儀及相關(guān)測(cè)序試劑盒等產(chǎn)品

咨詢(xún)illumina MiSeq測(cè)序試劑盒等產(chǎn)品歡迎您致電 上海易匯生物科技有限公司：1850 1609 238張經(jīng)理

"Illumina/Nextera DNA CD Indexes (24 Indexes, 24 Samples)/20018707/1 Ea

" Illumina 產(chǎn)品編號(hào)： 20018707美  元  價(jià)： $120.00會(huì)  員  價(jià)： 待定品       牌： Illumina產(chǎn)       地： 美國(guó)公       司： Illumina, Inc.產(chǎn)品分類(lèi)： 分子類(lèi)>二代測(cè)序>DNA文庫(kù)制備試劑盒公司分類(lèi)： Library Preparation Kits "Product Highlights:

The Nextera DNA Flex Library Prep Kit offers flexibility for many whole-genome sequencing applications.

Fastest Illumina library prep workflow, with ~3.5 hours total time

Flexibility to accommodate variations in sample type, DNA input amount, and application

Optimized library prep performance, generating reliable results

Save Time and Resources

The Nextera DNA Flex Library Prep Kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry reduces total library prep time to ~3.5 hours, from DNA extraction to library normalization.

Simplify Lab Operations

The Nextera DNA Flex workflow supports a broad DNA input range (1–500 ng), multiple sample types, and both small and large genomes. The workflow includes DNA extraction from blood, saliva, or dried blood spots*or bacterial colonies*. Gain the flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species, all with a single kit.

Obtain Reliable Results

While accommodating various study requirements, the Nextera DNA Flex workflow delivers consistent insert sizes, uniform coverage, and optimized performance, regardless of DNA input amount or genome size. The bead-based technology minimizes bias and opportunities for error, resulting in highly reproducIBLe sequencing data.

*Demonstrated protocols available.

Specifications:

Assay Time ~3.5 hours total assay time

Hands-On Time 1-1.5 hours

Input Quantity Small Genomes: 1-500 ng DNA; Large Genomes: 100-500 ng

Content Specifications Human Whole Genome, Small Whole Genome, Large Whole Genome

Mechanism of Action Bead-linked transposome

Multiplexing Up to 96 available indexes

Species Category Any Species

System Compatibility MiSeq,HiSeq X Ten,NextSeq 550,HiSeq 3000,HiSeq X Five,MiSeqDx in Research Mode,MiniSeq,HiSeq 2500,NovaSeq 6000,HiSeq 4000

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Method Whole-Genome Sequencing

Technology Sequencing

Automation CapABIlity Liquid Handling Robots"

illumina FC-410-1003 HiSeq 3000/4000 SBS Kit (300 cycles)

illumina FC-420-1001 MiniSeq High Output Reagent Kit (75-cycles)

illumina FC-420-1002 MiniSeq High Output Reagent Kit (150-cycles)

illumina FC-420-1003 MiniSeq High OutputReagent Kit (300-cycles)

illumina FC-420-1004 MiniSeq Mid Output Kit (300-cycles)

illumina FC-451-1001 ForenSeq Index Adapter Fixture

illumina FC-501-2501 HiSeq X Ten Reagent Kit v2.5

illumina FC-501-2521 HiSeq X Ten Reagent Kit v2.5 - 10 pack

illumina FC-502-2501 HiSeq X Five Reagent Kit v2.5

illumina FC-502-2521 HiSeq X Five Reagent Kit v2.5 - 10 pack

illumina FC-901-1001 Hybridization Manifold (0801-1320)

illumina FC-901-1002 Amplification Manifold (0801-1321)

illumina FC-901-1003 Cs, Bridge Manifold

illumina Nextera DNA Sample Preparation Kits

illumina FC-121-1031/1030/1012/1011

產(chǎn)品名稱(chēng)：llumina MiSeq Reagent Kit v2 (500-cycles)基因測(cè)序試劑盒

產(chǎn)品貨號(hào)：MS-102-2003

規(guī)格：

大輸出                              8.5 Gb（500個(gè)循環(huán)數(shù)MiSeq Reagent Kit v2）、5.1 Gb（300個(gè)循環(huán)數(shù)MiSeq Reagent Kit v2）、1.2 Gb（300個(gè)循環(huán)數(shù)MiSeq Reagent Micro Kit v2）、0.850 Gb（50個(gè)循環(huán)數(shù)MiSeq Reagent Kit v2）、0.5 Gb（500個(gè)循環(huán)數(shù)MiSeq Reagent Nano Kit v2）、0.3 Gb（300個(gè)循環(huán)數(shù)MiSeq Reagent Nano Kit v2）

每次運(yùn)行獲得的大read數(shù)    多達(dá)1500萬(wàn)

核酸類(lèi)型                             DNA, RNA

試劑類(lèi)型                             簇生成, 雙端測(cè)序, 邊合成邊測(cè)序

技術(shù)                                   測(cè)序

系統(tǒng)兼容性                         研究模式下的MiSeqDx ,研究模式下的MiSeq FGx, MiSeq , MiSeq

產(chǎn)品介紹：

MiSeq試劑盒v2實(shí)現(xiàn)更高通量，減少M(fèi)iSeq運(yùn)行次數(shù)。MiSeq試劑盒v2保留了與v1試劑盒相同的即用型預(yù)裝試劑盒，但改良了化學(xué)過(guò)程，提高簇密度，減少循環(huán)時(shí)間，并提高質(zhì)量(Q)分值。目前還提供Micro和Nano規(guī)格的MiSeq試劑盒v2，適合低通量的應(yīng)用。

改良化學(xué)過(guò)程后，循環(huán)時(shí)間更快、片段更長(zhǎng)、輸出更多

雙表面成像實(shí)現(xiàn)的片段數(shù)是v1試劑盒單表面成像的兩倍

使用500次循環(huán)的試劑盒可擴(kuò)展片段長(zhǎng)度

選擇適合您應(yīng)用的循環(huán)次數(shù)（50、300或500）

與升級(jí)的MiSeq系統(tǒng)結(jié)合使用，MiSeq試劑盒v2的雙表面成像可使每個(gè)流動(dòng)槽的通量翻倍。MiSeq試劑盒v2提供可生成較長(zhǎng)片段長(zhǎng)度的500次循環(huán)格式，以及廣受歡迎的50次循環(huán)和300次循環(huán)格式。

MiSeq試劑盒v2有標(biāo)準(zhǔn)、Micro和Nano三種配置，能滿(mǎn)足特定項(xiàng)目需求和可擴(kuò)展輸出量。

所有MiSeq試劑組件均采用RFID編碼，并與MiSeq系統(tǒng)智能交互，以驗(yàn)證與用戶(hù)定義的應(yīng)用程序的兼容性。

一個(gè)MiSeq試劑盒v3的也可以。它提供600循環(huán)格式，允許任何Illumina測(cè)序系統(tǒng)具有長(zhǎng)的讀取長(zhǎng)度，或150循環(huán)格式，可實(shí)現(xiàn)計(jì)數(shù)應(yīng)用。

該產(chǎn)品也可作為Illumina Advantage（TG）產(chǎn)品提供。Illumina Advantage大規(guī)模測(cè)序產(chǎn)品具有特定批次的出貨和測(cè)試，延長(zhǎng)的保質(zhì)期和*的變更通知，可提高實(shí)驗(yàn)室效率。

Illumina公司簡(jiǎn)介：

Illumina公司是由David Walt博士、CW 集團(tuán)的Larry Bock、獸醫(yī)學(xué)博士John Stuelpnagel、 Anthony Czarnik博士及Mark Chee博士于1998年4月共同組建，是遺傳變異和生物學(xué)功能分析領(lǐng)域的優(yōu)秀的產(chǎn)品、技術(shù)和服務(wù)供應(yīng)商。通過(guò)幫助客戶(hù)加快實(shí)現(xiàn)生物信息的采集、分析和應(yīng)用，來(lái)改善人類(lèi)健康。

背景知識(shí)：

全基因組測(cè)序概述

全基因組測(cè)序可謂是基因組zui為全面的研究方案?；蚪M信息已能用于鑒定遺傳疾病，查找驅(qū)使癌癥發(fā)展的突變，追蹤疾病的爆發(fā)。illumina迅速下降的測(cè)序成本以及處理大樣本數(shù)據(jù)能力的提升都使得如今的測(cè)序者可將全基因組測(cè)序視為基因組研究的zui強(qiáng)有力工具。

全基因組測(cè)序常被理解為用于測(cè)定人類(lèi)基因組，然而illumina新一代測(cè)序技術(shù)(NGS)的規(guī)模、靈活性體現(xiàn)于可以在任何物種上運(yùn)用測(cè)序技術(shù)，如農(nóng)業(yè)畜牧業(yè)，植物，或疾病相關(guān)微生物。

illumina全基因組測(cè)序的優(yōu)點(diǎn)

提供高分辨率、到逐個(gè)堿基的基因組視圖

可捕獲大的變異，以及小到可能被遺漏的變異

鑒定潛在的致病變異，從而進(jìn)行基因表達(dá)和調(diào)控機(jī)制的進(jìn)一步研究

在短時(shí)間內(nèi)提供大量的數(shù)據(jù)，以支持新基因組的組裝

呈現(xiàn)基因組視圖

外顯子組測(cè)序或靶向重測(cè)序等有側(cè)重點(diǎn)的方法只分析基因組的有限部分，全基因組測(cè)序則不同，能提供整個(gè)基因組的全面視圖。它是各種發(fā)現(xiàn)應(yīng)用——如鑒定致病變異和新基因組組裝——的理想選擇。全基因組測(cè)序可檢測(cè)單核苷酸變異、插入/缺失、拷貝數(shù)改變和大的結(jié)構(gòu)變異。隨著技術(shù)創(chuàng)新，的基因組測(cè)序儀能夠比以往更地開(kāi)展全基因組測(cè)序。

illumina主要的全基因組測(cè)序方法

大型全基因組測(cè)序

測(cè)序大型基因組（> 5 Mb），如人類(lèi)、植物或動(dòng)物基因組，為疾病和群體遺傳學(xué)研究提供寶貴信息。

小型全基因組測(cè)序

小型基因組測(cè)序（≤ 5 Mb）包括測(cè)序細(xì)菌、病毒或其他微生物的整個(gè)基因組。無(wú)需培養(yǎng)細(xì)菌，研究人員可利用NGS平行測(cè)序數(shù)千個(gè)小生物。

De Novo 測(cè)序

De novo測(cè)序是指在沒(méi)有參考序列的情況下，對(duì)一個(gè)新的基因組進(jìn)行測(cè)序。NGS能實(shí)現(xiàn)對(duì)任何物種的快速、準(zhǔn)確鑒定。

Phased基因組測(cè)序

Phased測(cè)序，又稱(chēng)基因組Phased。通過(guò)區(qū)分同源染色體的等位基因差異而獲得全基因組單體型，該信息通常對(duì)遺傳疾病的研究至關(guān)重要。

"Illumina/TruSeq Nano DNA LT Library Preparation Kit - Set A (12 Set A index tubes, 24 samples)/FC-121-4001/1 Ea

" Illumina 產(chǎn)品編號(hào)： FC-121-4001美  元  價(jià)： $735.00會(huì)  員  價(jià)： 待定品       牌： Illumina產(chǎn)       地： 美國(guó)公       司： Illumina, Inc.產(chǎn)品分類(lèi)： 分子類(lèi)>二代測(cè)序>DNA文庫(kù)制備試劑盒公司分類(lèi)： Library Preparation Kits "Product Highlights:

The TruSeq Nano DNA Library Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted library preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

Designed for low sample input

High coverage quality

Accelerated library preparation

Manual preparation of high-quality libraries in less than a day

The proven TruSeq DNA library preparation workflow has been streamlined by replacing gel-based size selection with bead-based selection, enabling researchers to prepare high-quality libraries in less than a day. It is optimized for a variety of read lengths, from 2 × 101 bp to 2 × 151 bp.

Use with limited DNA samples

The TruSeq Nano DNA protocol offers excellent results with as little as 100 ng input, eliminating the typical requirement for micrograms of DNA. This enables researchers to study samples with limited available DNA (eg, tumor samples) and helps preserve samples for use in future or alternate studies. In addition to accelerating the workflow, bead-based size selection avoids typical sample loss associated with gel-based selection.

Reduce library bias and coverage gaps

TruSeq Nano DNA kits reduce the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. These kits also provide excellent coverage of trADItionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables researchers to access more information from each sequencing run. TruSeq Nano DNA kits are validated for high-quality genomic coverage for virtually any next-generation sequencing application.

Access flexIBLe throughput options

Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

TruSeq Nano DNA LT Library Preparation Kits support 24-plex manual processing for low-throughput studies.

TruSeq Nano DNA HT Library Preparation Kits are 96-plex for high-throughput studies, and can be automated on liquid handling robots (or processed manually).

TruSeq Nano DNA Library Preparation Kits are also available for use with the automated NeoPrep Library Prep System.

Find an up-to-date list of automation vendors with robotic systems that support the HT library preparation kits

Specifications:

Assay Time 1 day

Hands-On Time 4 hours

Input Quantity 50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality

Content Specifications Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.

Multiplexing Up to 384 samples per sequencing run

Mechanism of Action Amplification

Method Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing

Variant Class Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)

Species Category Mammalian,Mouse,Human,Other,Rat,Plant

System Compatibility NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000

Specialized Sample Types Low Input

Technology Sequencing

Automation CapABIlity NeoPrep Digital Microfluidics,Liquid Handling Robots"

Illumina公司，致力于新一代測(cè)序和芯片技術(shù)的生產(chǎn)與開(kāi)發(fā)，提供產(chǎn)品與應(yīng)用資訊

Illumina公司創(chuàng)立于1998年4月，是遺傳變異和生物學(xué)功能分析領(lǐng)域的優(yōu)秀的產(chǎn)品、技術(shù)和服務(wù)供應(yīng)商。通過(guò)幫助客戶(hù)加快實(shí)現(xiàn)生物信息的采集、分析和應(yīng)用，來(lái)改善人類(lèi)健康。

Sequencing Kits/ Microarray Kits/Informatics Products/ Clinical Research Products/In Vitro Diagnostic/ Products/Molecular Biology Reagents/ Accessory Products

上海易匯生物科技有限公司于17年正式銷(xiāo)售Illumina公司基因芯片、二代測(cè)序NGS測(cè)序儀及相關(guān)測(cè)序試劑盒等產(chǎn)品

咨詢(xún)illumina MiSeq測(cè)序試劑盒等產(chǎn)品歡迎您致電 上海易匯生物科技有限公司：1850 1609 238張經(jīng)理

Illumina 代理 簇生成和測(cè)序試劑